Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35KD. The expected band size for SFTPA1 is at 26KD.
Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35KD. The expected band size for SFTPA1 is at 26KD.
Anti-SFTPA1 Antibody Picoband(r)
PA1347
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Rat
TargetSFTPA1
Overview
- SupplierBoster Bio
- Product NameAnti-SFTPA1 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.5-1 mg/ml
- FormulationLiquid
- Gene ID653509
- Target nameSFTPA1
- Target descriptionsurfactant protein A1
- Target synonyms35 kDa pulmonary surfactant-associated protein; alveolar proteinosis protein; COLEC4; collectin-4; PSAP; PSPA; PSP-A; pulmonary surfactant-associated protein A1; SFTP1; SFTPA1B; SPA; SP-A; SPA1; SP-A1; SP-A1 beta; SP-A1 delta; SP-A1 epsilon; SP-A1 gamma; surfactant protein A1B; surfactant, pulmonary-associated protein A1A; surfactant, pulmonary-associated protein A1B
- HostRabbit
- IsotypeIgG
- Protein IDQ8IWL2
- Protein NamePulmonary surfactant-associated protein A1
- Scientific DescriptionBoster Bio Anti-SFTPA1 Antibody catalog # PA1347. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Rat
- Reactivity SupplierHuman, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203