Figure 1. Western blot analysis of SGK1 using anti-SGK1 antibody (PB9434). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # PB9434) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 49 kDa. The expected band size for SGK1 is at 49 kDa.
Figure 1. Western blot analysis of SGK1 using anti-SGK1 antibody (PB9434). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # PB9434) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 49 kDa. The expected band size for SGK1 is at 49 kDa.
Anti-SGK1 Antibody Picoband(r)
PB9434
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human
TargetSGK1
Overview
- SupplierBoster Bio
- Product NameAnti-SGK1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for SGK1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID6446
- Target nameSGK1
- Target descriptionserum/glucocorticoid regulated kinase 1
- Target synonymsserine/threonine protein kinase SGK; serine/threonine-protein kinase Sgk1; SGK; Sgk1 variant i3
- HostRabbit
- IsotypeIgG
- Protein IDO00141
- Protein NameSerine/threonine-protein kinase Sgk1
- Scientific DescriptionBoster Bio Anti-SGK1 Antibody Picoband® catalog # PB9434. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203