Figure 1. Western blot analysis of SH2D3C using anti-SH2D3C antibody (A04497-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D3C antigen affinity purified polyclonal antibody (Catalog # A04497-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D3C at approximately 94 kDa. The expected band size for SH2D3C is at 94 kDa.
Figure 1. Western blot analysis of SH2D3C using anti-SH2D3C antibody (A04497-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH2D3C antigen affinity purified polyclonal antibody (Catalog # A04497-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH2D3C at approximately 94 kDa. The expected band size for SH2D3C is at 94 kDa.
Anti-SH2D3C Antibody Picoband(r)
A04497-1-PE
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSH2D3C
Overview
- SupplierBoster Bio
- Product NameAnti-SH2D3C Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID10044
- Target nameSH2D3C
- Target descriptionSH2 domain containing 3C
- Target synonymscas/HEF1-associated signal transducer; CHAT; chat-H; novel SH2-containing protein 3; NSP3; PRO34088; SH2 domain-containing Eph receptor-binding protein 1; SH2 domain-containing protein 3C; SHEP1
- HostRabbit
- IsotypeIgG
- Protein IDQ8N5H7
- Protein NameSH2 domain-containing protein 3C
- Scientific DescriptionBoster Bio Anti-SH2D3C Antibody Picoband® catalog # A04497-1. Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203