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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Anti-SH3BP2 Antibody

HPA036790
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSH3BP2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SH3BP2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6452
  • Target name
    SH3BP2
  • Target description
    SH3 domain binding protein 2
  • Target synonyms
    3BP-2, 3BP2, CRBM, CRPM, RES4-23, SH3 domain-binding protein 2, Abl-SH3 binding protein 2, TNFAIP3 interacting protein 2, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78314
  • Protein Name
    SH3 domain-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of 3BP2/SH3BP2 using anti-3BP2/SH3BP2 antibody (A04008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-3BP2/SH3BP2 antigen affinity purified polyclonal antibody (Catalog # A04008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 3BP2/SH3BP2 at approximately 70 kDa. The expected band size for 3BP2/SH3BP2 is at 62,70 kDa.
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Immunofluorescent staining of human cell line U2OS shows localization to the Golgi apparatus.
Product group Antibodies
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Anti-SH3BP2 AntibodyHPA036789
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TargetSH3BP2
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IHC image of CSB-PA021224LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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SH3BP2 AntibodyCSB-PA021224LA01HU
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