Anti-Shiga-like toxin 1 [13C4]

Absolute Antibody

Anti-Shiga-like toxin 1 [13C4]

7

The original IgG1 antibody neutralized the cytotoxicity of E. coli Shiga-like toxin (SLT) and were able to immunoprecipitate intact labeled toxin with Staphylococcus aureus protein A. The original IgG1 antibody neutralizes Shiga toxin from Shigella dysenteriae type 1 and Shiga-like toxic activities in crude cell extracts from Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus, and Salmonella typhimurium. The IgG1 version of this antibody was also used in a colony blot assay for the detection of bacterial colonies which produce high levels of SLT (PMID: 3905611). This antibody neutralizes the cytotoxic and lethal activities of Stx1, This antibody was also reported to block the binding of Stx1 to its receptor, globotriaosyl ceramide by binding three nonlinear regions of the molecule that span receptor recognition sites on StxB1, one of which includes the essential residue 55N. This antibody was able to identify histidine-tagged StxB1 in a western blot assay. It did not recognize Stx1d (PMID: 17030576). This antibody was used in a sandwich ELISA as a detector antibody to identify production of Stx1 by E. coli strain MHI 813 (PMID: 12734256).

ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

IP; neutralize; ELISA; WB

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203