Anti-Shiga-toxin 2 [11E10]

Absolute Antibody

Anti-Shiga-toxin 2 [11E10]

9

This antibody binds a conformational epitope on the A subunit of Shiga-like toxin 2 comprising region A (StxA2 residues 42 to 49), region B (StxA2 residues 96 to 100), and region C (StxA2 residues 244 to 259) (PMID: 19433543). This antibody does not bind

The binding characterization of the original IgG1 version of this antibody towards Shiga like toxin 2 was done using ELISA. The original IgG1 version of this antibody was also used to immunoprecipitate SLT-Il toxoid. This antibody is capable of neutralizing the cytotoxicity of SLT-ll but not of SLT-I, Shiga toxin, or a variant of SLT-II produced by E. coli that causes edema disease of swine (PMID: 3053764). This antibody was also used a primary antibody in the detection of SLT-II in a western blot assay. It was reported that binding of 11E10 to Stx2 prevented the toxin from inhibiting protein synthesis in an in vitro assay but also altered the overall cellular distribution of Stx2 in Vero cells. It was suggested that binding of antibody 11E10 to Stx2 neutralizes the effects of the toxin by preventing the toxin from reaching and/or inactivating the ribosomes (PMID: 19433543). Administration of original mouse version of this antibody in a hemolytic-uremic syndrome (HUS) mouse model resulted in full recovery of mice after 4 days and the mice displayed normal renal function and normal levels of neutrophils and lymphocytes. These mice showed decreased fibrin(ogen) deposition and expression of proinflammatory mediators compared to those of Stx2-treated mice in the absence of antibody. It was concluded that passive immunization of mice with anti-Stx2 antibody prevented the lethal effects of Stx2 (PMID: 18694970). Another study reported that mice orally intoxicated with the original mouse version of this antibody got full protection and that orally intoxicated mice could be rescued by antibody 11E10 6 h but not 24 h after Stx2a delivery (PMID: 24379294).

ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

11E10

stxA2

Shiga toxin Stx2c subunit A

L0103; Shiga toxin Stx2c subunit A; sltIIA; ST933Wp40; stx2A

Rabbit

IgG

Shiga-like toxin 2 subunit A

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203