Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (A18878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # A18878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD.
Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (A18878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # A18878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD.
Anti-SHISA7 Antibody Picoband(r)
A18878-1-FITC
TargetSHISA7
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-SHISA7 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID729956
- Target nameSHISA7
- Target descriptionshisa family member 7
- Target synonymsCKAMP59; cystine-knot AMPAR modulating protein of 59 kDa; GABA(A) receptor auxiliary subunit Shisa7; protein shisa-7; shisa homolog 7; UPF0626 protein A
- Protein IDA6NL88
- Protein NameProtein shisa-7
- Scientific DescriptionBoster Bio Anti-SHISA7 Antibody Picoband® catalog # A18878-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203