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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.

Anti-SHROOM1 Antibody

HPA037690
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHROOM1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SHROOM1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID134549
  • Target name
    SHROOM1
  • Target description
    shroom family member 1
  • Target synonyms
    APXL2, protein Shroom1, apical protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ2M3G4
  • Protein Name
    Protein Shroom1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SHROOM1 using anti-SHROOM1 antibody (A12817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHROOM1 antigen affinity purified polyclonal antibody (Catalog # A12817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHROOM1 at approximately 91 kDa. The expected band size for SHROOM1 is at 91 kDa.
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