Anti-Sigma1 [5C6]

Absolute Antibody

Anti-Sigma1 [5C6]

9

The structure of 5C6 against Sigma1 was solved crystallographically to 3.7 angstrom resolution (Dietrich et al, 2017). The 5C6 Fab regions each contact the upper part of the Sigma1 head, which forms a beta-spiral repeat structure. The VH CDRs contact the

5C6 was shown recently to block reoviral infection of L929 mouse fibroblasts in a dose-dependent manner, using immunofluorescence staining (Dietrich et al, 2017). Fibroblasts were incubated with virus-antibody mixtures, and immunofluorescence staining of the cells enabled detection of infectivity. 5C6 is highly specific for the T1L reoviral strain, as opposed to the T3D strain which was also tested. 5C6 was also used to immunoprecipitate reoviral particles.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

5C6

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203