Anti-SIRP alpha [SAF10.1]

Absolute Antibody

Anti-SIRP alpha [SAF10.1]

7

This antibody recognises an epitope in the membrane most distal domain (D1) of SIRP alpha. It crossreacts with other SIRPs.

SAF10.1, can be used to detect SIRPs in a wide variety of tissues and cell types. For instance, this antibody was successfully used to detect SIRPs from COS7.2 cell lysates via Western blot (Lee et al., 2008).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, Other Application

WB; IF; IP; FC; binding assay

Research Use Only

Monoclonal

SAF10.1

SIRPA

signal regulatory protein alpha

BIT; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172A; inhibitory receptor SHPS-1; macrophage fusion receptor; MFR; MYD-1; myd-1 antigen; P84; PTPNS1; SHPS1; SIRP; tyrosine phosphatase SHP substrate 1; tyrosine-protein phosphatase non-receptor type substrate 1

Human

IgG1

Tyrosine-protein phosphatase non-receptor type substrate 1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antibody was raised by immunisisng BALB/c mice with an eukaryotically expressed SIRPalfa-Fc fusion protein consisting of the extracellular domain and emulsified with complete Freund’s adjuvant and followed by two subsequent boosts of SIRPalfa-Fc fusion protein emulsified with IFA (Lee et al., 2007).

-20°C,2°C to 8°C

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