More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband(r)
Figure 1. Western blot analysis of GLUT5/SLC2A5 using anti-GLUT5/SLC2A5 antibody (PA1737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT5/SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PA1737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT5/SLC2A5 at approximately 50 kDa. The expected band size for GLUT5/SLC2A5 is at 55 kDa.
Figure 1. Western blot analysis of GLUT5/SLC2A5 using anti-GLUT5/SLC2A5 antibody (PA1737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT5/SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PA1737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT5/SLC2A5 at approximately 50 kDa. The expected band size for GLUT5/SLC2A5 is at 55 kDa.
Figure 1. Western blot analysis of GLUT5/SLC2A5 using anti-GLUT5/SLC2A5 antibody (PA1737). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT5/SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PA1737) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT5/SLC2A5 at approximately 50 kDa. The expected band size for GLUT5/SLC2A5 is at 55 kDa.

Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband(r)

Research Use Only
PA1737
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityMouse, Rat
TargetSlc2a5
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SLC2A5 Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    IHP, IHF, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Formulation
    Lyophilized
  • Gene ID56485
  • Target name
    Slc2a5
  • Target description
    solute carrier family 2 (facilitated glucose transporter), member 5
  • Target synonyms
    AI526984; fructose transporter; glucose transporter type 5, small intestine; GLUT; Glut5; GLUT-5; Slc5a; solute carrier family 2, facilitated glucose transporter member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9WV38
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 5
  • Scientific Description
    Boster Bio Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody catalog # PA1737. Tested in IHC, IHC-F, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203