Immunohistochemical analysis of paraffin-embedded human kidney, using Smad1 Antibody(M00728-1) SMAD1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD1 Antibody (M00728-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Smad1 Rabbit Monoclonal Antibody
M00728-1
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSMAD1
Overview
- SupplierBoster Bio
- Product NameAnti-Smad1 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCAC-19
- FormulationLiquid
- Gene ID4086
- Target nameSMAD1
- Target descriptionSMAD family member 1
- Target synonymsBSP1; BSP-1; JV41; JV4-1; MAD, mothers against decapentaplegic homolog 1; MADH1; MADR1; Mad-related protein 1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ15797
- Protein NameMothers against decapentaplegic homolog 1
- Scientific DescriptionBoster Bio Anti-Smad1 Rabbit Monoclonal Antibody catalog # M00728-1. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
- ReactivityHuman, Mouse
- Storage Instruction-20°C
- UNSPSC12352203