Bio-Connect
Figure 1. Western blot analysis of SND1 using anti-SND1 antibody (M02602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat pancrease tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SND1 antigen affinity purified monoclonal antibody (Catalog # M02602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SND1 at approximately 110 kDa. The expected band size for SND1 is at 102 kDa.
Figure 1. Western blot analysis of SND1 using anti-SND1 antibody (M02602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat pancrease tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SND1 antigen affinity purified monoclonal antibody (Catalog # M02602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SND1 at approximately 110 kDa. The expected band size for SND1 is at 102 kDa.
Figure 1. Western blot analysis of SND1 using anti-SND1 antibody (M02602-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human U87 whole cell lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat pancrease tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancrease tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SND1 antigen affinity purified monoclonal antibody (Catalog # M02602-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SND1 at approximately 110 kDa. The expected band size for SND1 is at 102 kDa.

Anti-SND1 Antibody Picoband(r) (monoclonal, 5F5E9)

M02602-1-IFLUOR647
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSND1
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SND1 Antibody Picoband(r) (monoclonal, 5F5E9)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5F5E9
  • Concentration
    500 ug/ml
  • Conjugate
    Other Conjugate
  • Gene ID27044
  • Target name
    SND1
  • Target description
    staphylococcal nuclease and tudor domain containing 1
  • Target synonyms
    EBNA2 coactivator p100; p100; staphylococcal nuclease domain-containing protein 1; TDRD11; testis tissue sperm-binding protein Li 82P; tudor domain-containing protein 11; Tudor-SN
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ7KZF4
  • Protein Name
    Staphylococcal nuclease domain-containing protein 1
  • Scientific Description
    Boster Bio Anti-SND1 Antibody Picoband® (monoclonal, 5F5E9) catalog # M02602-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203