
Figure 1. Western blot analysis of SOX17 using anti-SOX17 antibody (A01665-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX17 antigen affinity purified polyclonal antibody (Catalog # A01665-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX17 at approximately 45 kDa. The expected band size for SOX17 is at 45 kDa.
Anti-SOX17 Antibody Picoband(r)
A01665-2-HRP
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSOX17
Overview
- SupplierBoster Bio
- Product NameAnti-SOX17 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID64321
- Target nameSOX17
- Target descriptionSRY-box transcription factor 17
- Target synonymsSRY (sex determining region Y)-box 17; SRY-box 17; SRY-related HMG-box transcription factor SOX17; transcription factor SOX-17; VUR3
- HostRabbit
- IsotypeIgG
- Protein IDQ9H6I2
- Protein NameTranscription factor SOX-17
- Scientific DescriptionBoster Bio Anti-SOX17 Antibody Picoband® catalog # A01665-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203