Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: 293T Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: 293T Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
Anti-SOX4 Antibody Picoband(r)
PB9618
ApplicationsWestern Blot
Product group Antibodies
ReactivityHamster, Human, Mouse
TargetSOX4
Overview
- SupplierBoster Bio
- Product NameAnti-SOX4 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID6659
- Target nameSOX4
- Target descriptionSRY-box transcription factor 4
- Target synonymsCSS10; ecotropic viral integration site 16; EVI16; SRY (sex determining region Y)-box 4; SRY-box 4; SRY-related HMG-box gene 4; transcription factor SOX-4
- HostRabbit
- IsotypeIgG
- Protein IDQ06945
- Protein NameTranscription factor SOX-4
- Scientific DescriptionBoster Bio Anti-SOX4 Antibody Picoband® catalog # PB9618. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203