Figure 1. Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84 kDa. The expected band size for SOX5 is at 84 kDa.
Figure 1. Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84 kDa. The expected band size for SOX5 is at 84 kDa.
Anti-SOX5 Antibody Picoband(r)
PB9507-CY3
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Human, Mouse, Porcine, Rat
TargetSOX5
Overview
- SupplierBoster Bio
- Product NameAnti-SOX5 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID6660
- Target nameSOX5
- Target descriptionSRY-box transcription factor 5
- Target synonymsLAMSHF; L-SOX5; L-SOX5B; L-SOX5F; SRY (sex determining region Y)-box 5; SRY-box 5; transcription factor SOX-5
- HostRabbit
- IsotypeIgG
- Protein IDP35711
- Protein NameTranscription factor SOX-5
- Scientific DescriptionBoster Bio Anti-SOX5 Antibody Picoband® catalog # PB9507. Tested in WB applications. This antibody reacts with Human, Mouse, Pig, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse, Porcine, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203