Figure 1. Western blot analysis of SP5 using anti-SP5 antibody (PB9443). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP5 antigen affinity purified polyclonal antibody (Catalog # PB9443) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP5 at approximately 42 kDa. The expected band size for SP5 is at 42 kDa.
Figure 1. Western blot analysis of SP5 using anti-SP5 antibody (PB9443). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP5 antigen affinity purified polyclonal antibody (Catalog # PB9443) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP5 at approximately 42 kDa. The expected band size for SP5 is at 42 kDa.
Anti-Sp5 Antibody Picoband(r)
PB9443-BIOTIN
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human
TargetSP5
Overview
- SupplierBoster Bio
- Product NameAnti-Sp5 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for Sp5 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID389058
- Target nameSP5
- Target descriptionSp5 transcription factor
- Target synonymstranscription factor Sp5
- HostRabbit
- IsotypeIgG
- Protein IDQ6BEB4
- Protein NameTranscription factor Sp5
- Scientific DescriptionBoster Bio Anti-Sp5 Antibody Picoband® catalog # PB9443. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203