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Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows moderate nuclear positivity in glandular cells.

Anti-SPIDR Antibody

HPA027201
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPIDR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPIDR Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23514
  • Target name
    SPIDR
  • Target description
    scaffold protein involved in DNA repair
  • Target synonyms
    KIAA0146, ODG9, DNA repair-scaffolding protein, scaffolding protein involved in DNA repair
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14159
  • Protein Name
    DNA repair-scaffolding protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPIDR using anti-SPIDR antibody (A04260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPIDR antigen affinity purified polyclonal antibody (Catalog # A04260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPIDR at approximately 95 kDa. The expected band size for SPIDR is at 100 kDa.
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
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