Anti-Spike protein [Ty1]

Absolute Antibody

Anti-Spike protein [Ty1]

9

This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2. Ty1 binds to an epitope on the RBD accessible in both the up and down conformations and that Ty1 sterically hinders RBD-ACE2 binding.

This alpaca-derived single domain antibody targets the RBD of the SARS-CoV-2 spike glycoprotein and potently neutralizes the virus. Ty1 successfully neutralized SARS-CoV-2 pseudotyped viruses in vitro at an IC50 of 0.77 microg/ml (54 nM). The binding specificity of Ty1 to spike protein was determined by flow cytometry. Ty1 was also able to recognize the virus infected Vero E6 cells in an immunofluorescence assay. It is also reported that Ty1 recognizes and binds the viral spike protein with high specificity in its native conformation in SARS-CoV-2 infected cells. RBD bound to surface-immobilized Ty1 with high affinity in the 5-50 nM range in Biolayer Interferometry (Hanke et al., 2020).

Flow Cytometry, ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

Ty1

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Mouse

IgG1

Spike glycoprotein

This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203