Figure 1. Western blot analysis of SPINK13 using anti-SPINK13 antibody (A18284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK13 antigen affinity purified polyclonal antibody (Catalog # A18284) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPINK13 at approximately 24 kDa. The expected band size for SPINK13 is at 11 kDa.
Figure 1. Western blot analysis of SPINK13 using anti-SPINK13 antibody (A18284). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPINK13 antigen affinity purified polyclonal antibody (Catalog # A18284) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPINK13 at approximately 24 kDa. The expected band size for SPINK13 is at 11 kDa.
Anti-SPINK13 Antibody Picoband(r)
A18284-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSPINK13
Overview
- SupplierBoster Bio
- Product NameAnti-SPINK13 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID153218
- Target nameSPINK13
- Target descriptionserine peptidase inhibitor Kazal type 13
- Target synonymsHBVDNAPTP1; Hepatitis B Virus (HBV) DNA polymerase transactivated protein 1; hepatitis B virus DNA polymerase transactivated human serine protease inhibitor; hepatitis B virus DNA polymerase transactivated serine protease inhibitor; HESPINTOR; LiESP6; serine peptidase inhibitor, Kazal type 13 (putative); serine protease inhibitor Kazal-type 13; serine protease inhibitor Kazal-type 5-like 3; SPINK5L3
- HostRabbit
- IsotypeIgG
- Protein IDQ1W4C9
- Protein NameSerine protease inhibitor Kazal-type 13
- Scientific DescriptionBoster Bio Anti-SPINK13 Antibody Picoband® catalog # A18284. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203