Western blot analysis of STAU1 using anti-STAU1 antibody (M04259) in K562 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAU1 antigen affinity purified polyclonal antibody (Catalog # M04259) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Western blot analysis of STAU1 using anti-STAU1 antibody (M04259) in K562 cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAU1 antigen affinity purified polyclonal antibody (Catalog # M04259) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-Staufen STAU1 Monoclonal Antibody
M04259
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAU1
Overview
- SupplierBoster Bio
- Product NameAnti-Staufen Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDACAC-19
- FormulationLiquid
- Gene ID6780
- Target nameSTAU1
- Target descriptionstaufen double-stranded RNA binding protein 1
- Target synonymsdouble-stranded RNA-binding protein Staufen homolog 1; PPP1R150; protein phosphatase 1, regulatory subunit 150; STAU; staufen, RNA binding protein, homolog 1
- HostRabbit
- IsotypeIgG
- Protein IDO95793
- Protein NameDouble-stranded RNA-binding protein Staufen homolog 1
- Scientific DescriptionBoster Bio Anti-Staufen STAU1 Monoclonal Antibody catalog # M04259. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203