Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Anti-SUFU Picoband(r) Antibody
A02279-1-APC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSUFU
Overview
- SupplierBoster Bio
- Product NameAnti-SUFU Picoband(r) Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID51684
- Target nameSUFU
- Target descriptionSUFU negative regulator of hedgehog signaling
- Target synonymsJBTS32; negative regulator of hedgehog signaling; PRO1280; SUFUH; SUFUXL; suppressor of fused homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ9UMX1
- Protein NameSuppressor of fused homolog
- Scientific DescriptionBoster Bio Anti-SUFU Picoband® Antibody catalog # A02279-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203