Anti-Sulphonamide drug [4D11], Human IgG1, Fc Silent(tm), kappa

Absolute Antibody

Anti-Sulphonamide drug [4D11], Human IgG1, Fc Silent(tm), kappa

9

This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulf

The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL-1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).

ELISA

Research Use Only

Monoclonal

4D11

Human

IgG1

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.

Other Species

-20°C,2°C to 8°C

12352203