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Figure 1. Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.
Figure 1. Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.
Figure 1. Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.

Anti-SULT2A1 Antibody Picoband(r)

Research Use Only
PB9821-10UG
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSULT2A1
10 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-SULT2A1 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID6822
  • Target name
    SULT2A1
  • Target description
    sulfotransferase family 2A member 1
  • Target synonyms
    alcohol/hydroxysteroid sulfotransferase; bile-salt sulfotranasferase 2A1; DHEAS; DHEA-ST; DHEA-ST8; HST; hSTa; ST2; ST2A1; ST2A3; STD; sulfotransferase 2A1; sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1; SULT2A3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06520
  • Protein Name
    Bile salt sulfotransferase
  • Scientific Description
    Boster Bio Anti-SULT2A1 Antibody Picoband® catalog # PB9821. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203