
Figure 1. Western blot analysis of CCS using anti-CCS antibody (A00314-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: rat spleen tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCS antigen affinity purified polyclonal antibody (Catalog # A00314-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCS at approximately 34KD. The expected band size for CCS is at 29KD.
Anti-Superoxide Dismutase 4/CCS Antibody Picoband(r)
A00314-1-10UG
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCS
Overview
- SupplierBoster Bio
- Product NameAnti-Superoxide Dismutase 4/CCS Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID9973
- Target nameCCS
- Target descriptioncopper chaperone for superoxide dismutase
- Target synonymscopper chaperone for superoxide dismutase; superoxide dismutase copper chaperone
- HostRabbit
- IsotypeIgG
- Protein IDO14618
- Protein NameCopper chaperone for superoxide dismutase
- Scientific DescriptionBoster Bio Anti-Superoxide Dismutase 4/CCS Antibody Picoband® catalog # A00314-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203