Figure 2. IHC analysis of Synapsin I using anti-Synapsin I antibody (PB10099). Synapsin I was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Synapsin I Antibody (PB10099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Synapsin I using anti-Synapsin I antibody (PB10099). Synapsin I was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Synapsin I Antibody (PB10099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Synapsin I/SYN1 Antibody Picoband(r)
PB10099-FITC
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
TargetSYN1
Overview
- SupplierBoster Bio
- Product NameAnti-Synapsin I/SYN1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID6853
- Target nameSYN1
- Target descriptionsynapsin I
- Target synonymsbrain protein 4.1; EPILX; MRX50; SYN1a; SYN1b; synapsin Ib; synapsin-1; SYNI
- HostRabbit
- IsotypeIgG
- Protein IDP17600
- Protein NameSynapsin-1
- Scientific DescriptionBoster Bio Anti-Synapsin I/SYN1 Antibody Picoband® catalog # PB10099. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Rabbit, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203