Figure 2. Flow Cytometry analysis of A549 cells using anti-SynCAM antibody (PB9136). Overlay histogram showing A549 cells stained with PB9136 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SynCAM Antibody (PB9136, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 2. Flow Cytometry analysis of A549 cells using anti-SynCAM antibody (PB9136). Overlay histogram showing A549 cells stained with PB9136 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SynCAM Antibody (PB9136, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Anti-SynCAM/CADM1 Antibody Picoband(r)
PB9136-APC
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetCADM1
Overview
- SupplierBoster Bio
- Product NameAnti-SynCAM/CADM1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for SynCAM is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID23705
- Target nameCADM1
- Target descriptioncell adhesion molecule 1
- Target synonymsBL2; cell adhesion molecule 1; IGSF4; IGSF4A; immunoglobulin superfamily member 4; immunoglobulin superfamily, member 4D variant 1; immunoglobulin superfamily, member 4D variant 2; NECL2; Necl-2; nectin-like 2; nectin-like protein 2; RA175; sgIGSF; spermatogenic immunoglobulin superfamily; ST17; sTSLC-1; synaptic cell adhesion molecule; SYNCAM; synCAM1; truncated CADM1 protein; TSLC1; TSLC-1; TSLC1/Nectin-like 2/IGSF4; tumor suppressor in lung cancer 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9BY67
- Protein NameCell adhesion molecule 1
- Scientific DescriptionBoster Bio Anti-SynCAM/CADM1 Antibody Picoband® catalog # PB9136. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203