Figure 1. Western blot analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB9743). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HT1080 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate, Lane 3: 293T Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1 antigen affinity purified polyclonal antibody (Catalog # PB9743) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1 at approximately 50 kDa. The expected band size for Syndecan-1 is at 32 kDa.
Figure 1. Western blot analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB9743). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HT1080 Whole Cell Lysate, Lane 2: MCF-7 Whole Cell Lysate, Lane 3: 293T Whole Cell Lysate, Lane 4: HEPG2 Whole Cell Lysate, Lane 5: HELA Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1 antigen affinity purified polyclonal antibody (Catalog # PB9743) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1 at approximately 50 kDa. The expected band size for Syndecan-1 is at 32 kDa.
Anti-Syndecan-1/SDC1 Antibody Picoband(r)
PB9743-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSDC1
Overview
- SupplierBoster Bio
- Product NameAnti-Syndecan-1/SDC1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID6382
- Target nameSDC1
- Target descriptionsyndecan 1
- Target synonymsCD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; SDC; SYND1; syndecan; syndecan proteoglycan 1; syndecan-1
- HostRabbit
- IsotypeIgG
- Protein IDP18827
- Protein NameSyndecan-1
- Scientific DescriptionBoster Bio Anti-Syndecan-1/SDC1 Antibody Picoband® catalog # PB9743. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203