Figure 1. Western blot analysis of SynDIG4/PRRT1 using anti-SynDIG4/PRRT1 antibody (A13564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SynDIG4/PRRT1 antigen affinity purified polyclonal antibody (Catalog # A13564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SynDIG4/PRRT1 at approximately 36 kDa. The expected band size for SynDIG4/PRRT1 is at 36 kDa.
Figure 1. Western blot analysis of SynDIG4/PRRT1 using anti-SynDIG4/PRRT1 antibody (A13564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SynDIG4/PRRT1 antigen affinity purified polyclonal antibody (Catalog # A13564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SynDIG4/PRRT1 at approximately 36 kDa. The expected band size for SynDIG4/PRRT1 is at 36 kDa.
Anti-SynDIG4/PRRT1 Antibody Picoband(r)
A13564-1-APC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRRT1
Overview
- SupplierBoster Bio
- Product NameAnti-SynDIG4/PRRT1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID80863
- Target namePRRT1
- Target descriptionproline rich transmembrane protein 1
- Target synonymsC6orf31; dispanin subfamily D member 1; DSPD1; IFITMD7; interferon induced transmembrane protein domain containing 7; NG5; proline-rich transmembrane protein 1; synapse differentiation-induced protein 4; SynDIG4
- HostRabbit
- IsotypeIgG
- Protein IDQ99946
- Protein NameProline-rich transmembrane protein 1
- Scientific DescriptionBoster Bio Anti-SynDIG4/PRRT1 Antibody Picoband® catalog # A13564-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203