Anti-Tau [MN423]

Absolute Antibody

Anti-Tau [MN423]

9

This antibody is specific for the pronase-resistant core PHF and the 12 kDa tau fragment. It does not recognize normal full-length tau. It is also reported that this antibody specifically recognizes tau truncated at Glu391 on the C terminus side. The addi

The original format of this antibody was characterized in detail (Novak et al., 1989; PMID: 2602435) and has been extensively utilized in Alzheimers disease research: It played a crucial role in the structural characterization of the Alzheimer paired helical filament (PHF) core (Wischik et al., 1988; PMID: 2455299), and was employed for extracting two peptide fragments (9.5 and 12 kDa) of the PFH pronase-resistant core through immunoblotting (Wischik et al. 1988; PMID: 3132715). Additionally, it was used to study the folding of the Alzheimers core PHF subunit through competitive ELISA and western blotting (Skrabana et al., 2004; PMID: 15196943). It has been instrumental in the molecular investigation of neurofibrillary degeneration in Alzheimers disease (Bondareff et al., 1994; PMID: 7509849). Epitope mapping revealed that it requires a glycine at position -3 with respect to the C terminus (Khuebachova et al., 2002; PMID: 11983234). In the realm of tau protein studies related to Alzheimers disease, it has been employed for immunohistochemistry on brain tissue samples (Mondragon-Rodriguez et al., 2014; PMID: 24033439). Double and triple-labeled immunofluorescence was conducted using this antibody on brain tissue from Alzheimers disease patients (Mondragon-Rodriguez et al., 2014; PMID: 24033439) (Garcia-Sierra et al., 2003; PMID: 12719624). This antibody was used to immunoblot purified candidate tau analogs (Novak et al., 1993; PMID: 7679073). Furthermore, it was employed in immunohistochemical staining of brain tissue from patients with Alzheimers disease, NCI, PSP, or CBD (Berry et al., 2004; PMID: 15475684). Western blot analysis on brain tissue from Alzheimers patients was performed (Fitzpatrick et al., 2017; PMID: 28678775). This antibody was also used for a competitive ELISA on the Alzheimers disease expression cDNA library made from the hippocampus of a 70-year-old patient who died from Alzheimers disease (Khuebachova et al., 2002; PMID: 11983234).

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

Research Use Only

Monoclonal

MN423

MAPT

microtubule associated protein tau

DDPAC; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPTL; microtubule-associated protein tau; MSTD; MTBT1; MTBT2; neurofibrillary tangle protein; paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; tau-40

Mouse

IgG1

Microtubule-associated protein tau

This reformatted mouse antibody was made using the variable domain sequences of the original mouse IgG2b format for improved compatibility with existing reagents, assays, and techniques.

Human

-20°C,2°C to 8°C

12352203