Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CCT7 antibody (A08169-2). Overlay histogram showing HepG2 cells stained with A08169-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT7 Antibody (A08169-2,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-TCP1 eta/CCT7 Antibody Picoband(r)
A08169-2-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCT7
Overview
- SupplierBoster Bio
- Product NameAnti-TCP1 eta/CCT7 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID10574
- Target nameCCT7
- Target descriptionchaperonin containing TCP1 subunit 7
- Target synonymsCCTETA; CCT-eta; CCTH; chaperonin containing t-complex polypeptide 1, eta subunit; HIV-1 Nef interacting protein; NIP7-1; T-complex protein 1 subunit eta; TCP1ETA; TCP-1-eta
- HostRabbit
- IsotypeIgG
- Protein IDQ99832
- Protein NameT-complex protein 1 subunit eta
- Scientific DescriptionBoster Bio Anti-TCP1 eta/CCT7 Antibody Picoband® catalog # A08169-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203