Anti-Tetanus toxoid [9F12]

Absolute Antibody

Anti-Tetanus toxoid [9F12]

7

This antibody binds the intact or wild type tetanus toxin of Clostridium tetani. It was also reported to bind the tetanus toxin fragment B. Tetanus toxin is an extremely potent neurotoxin produced by Clostridium tetani.

The screening of hybridoma supernatants to identify binding towards tetanus toxin was done using ELISA. The protective capacity of this antibody against tetanus toxoid was tested using the mouse tetanus toxin neutralization assay. The mice which received the tetanus toxin preincubated with 9F12 were completely protected (PMID: 7174795). The original antibody was used to study the role of gangliosides in the binding and internalization of bacterial toxins (PMID: 11093135).

Functional Assay, ELISA, Neutralisation/Blocking

ELISA; neutralize; functional assay

Research Use Only

Monoclonal

9F12

tetX

tetanus neurotoxin TetX

CTC_p60; CTC_RS14060; pE88_60; tetanus neurotoxin TetX

Rabbit

IgG

Tetanus toxin

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Bacteria

Clostridium tetani

The original antibody was generated from the peripheral blood mononuclear cells form human donors after immunization with tetanus toxoids and fusing these lymphocytes with mouse myeloma line P3-X63-Ag 8.653.

-20°C,2°C to 8°C

12352203