Figure 1. Western blot analysis of TFPI2 using anti-TFPI2 antibody (A03697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: HELA whole Cell lysates, Lane 3: human placenta tissue lysates, Lane 4: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI2 antigen affinity purified polyclonal antibody (Catalog # A03697-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI2 at approximately 27KD, 35KD. The expected band size for TFPI2 is at 27KD.
Figure 1. Western blot analysis of TFPI2 using anti-TFPI2 antibody (A03697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: HELA whole Cell lysates, Lane 3: human placenta tissue lysates, Lane 4: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI2 antigen affinity purified polyclonal antibody (Catalog # A03697-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI2 at approximately 27KD, 35KD. The expected band size for TFPI2 is at 27KD.
Anti-TFPI2 Antibody Picoband(r)
A03697-1
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse
TargetTFPI2
Overview
- SupplierBoster Bio
- Product NameAnti-TFPI2 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- Applications SupplierWB, IHP, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID7980
- Target nameTFPI2
- Target descriptiontissue factor pathway inhibitor 2
- Target synonymsplacental protein 5; PP5; REF1; retinal pigment epithelium cell factor 1; TFPI-2; tissue factor pathway inhibitor 2
- HostRabbit
- IsotypeIgG
- Protein IDP48307
- Protein NameTissue factor pathway inhibitor 2
- Scientific DescriptionBoster Bio Anti-TFPI2 Antibody Picoband® catalog # A03697-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse
- Reactivity SupplierHuman, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203