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Figure 1. Western blot analysis of TIF1 gamma/TRIM33 using anti-TIF1 gamma/TRIM33 antibody (M03133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TIF1 gamma/TRIM33 antigen affinity purified monoclonal antibody (Catalog # M03133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TIF1 gamma/TRIM33 at approximately 150KD. The expected band size for TIF1 gamma/TRIM33 is at 150KD.
Figure 1. Western blot analysis of TIF1 gamma/TRIM33 using anti-TIF1 gamma/TRIM33 antibody (M03133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TIF1 gamma/TRIM33 antigen affinity purified monoclonal antibody (Catalog # M03133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TIF1 gamma/TRIM33 at approximately 150KD. The expected band size for TIF1 gamma/TRIM33 is at 150KD.
Figure 1. Western blot analysis of TIF1 gamma/TRIM33 using anti-TIF1 gamma/TRIM33 antibody (M03133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TIF1 gamma/TRIM33 antigen affinity purified monoclonal antibody (Catalog # M03133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TIF1 gamma/TRIM33 at approximately 150KD. The expected band size for TIF1 gamma/TRIM33 is at 150KD.

Anti-TIF1 gamma/TRIM33 Antibody Picoband(r) (monoclonal, 8I8)

M03133-2-FITC
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM33
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TIF1 gamma/TRIM33 Antibody Picoband(r) (monoclonal, 8I8)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8I8
  • Concentration
    500 ug/ml
  • Conjugate
    FITC
  • Gene ID51592
  • Target name
    TRIM33
  • Target description
    tripartite motif containing 33
  • Target synonyms
    E3 ubiquitin-protein ligase TRIM33; ECTO; ectodermin homolog; protein Rfg7; PTC7; RET-fused gene 7 protein; RFG7; RING-type E3 ubiquitin transferase TRIM33; TF1G; TIF1G; TIF1GAMMA; TIF1-gamma; TIFGAMMA; transcriptional intermediary factor 1 gamma
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ9UPN9
  • Protein Name
    E3 ubiquitin-protein ligase TRIM33
  • Scientific Description
    Boster Bio Anti-TIF1 gamma/TRIM33 Antibody Picoband® (monoclonal, 8I8) catalog # M03133-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203