Anti-TIGIT [MT251]

Absolute Antibody

Anti-TIGIT [MT251]

7

The original antibody is specific for TIGIT. The antibody does not cross-react with LAG-3, PD-1 and PD-L1.

The original mouse antibody was subjected to humanized modification, keeping a similar binding activity. The specificity of the original and the humanized format of the antibody was confirmed by ELISA analysis (EC50= 0.09901 microg/mL and 0.07412 microg/mL respectively). The original format of antibody blocked the binding activity of TIGIT and CD155. The original format and the humanized version of the antibody could recognize the TIGIT antigen expressed by Jurkat/TIGIT cells by FACS analysis. The original format of the antibody was able to effectively block the binding of TIGIT to CD155 (CN112794909).

Flow Cytometry, ELISA, Neutralisation/Blocking

ELISA; FACS; blocking

Research Use Only

Monoclonal

MT251

TIGIT

T cell immunoreceptor with Ig and ITIM domains

T-cell immunoreceptor with Ig and ITIM domains; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSIG9, VSTM3; VSTM3; Washington University cell adhesion molecule; WUCAM

Rabbit

IgG

T-cell immunoreceptor with Ig and ITIM domains

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by immunizing BALB/c mice with recombinant human TIGIT protein.

-20°C,2°C to 8°C

12352203