Anti-Tim-1 [3B3]

Absolute Antibody

Anti-Tim-1 [3B3]

9

This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1 has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1 is involved in renal injury, tissue h

This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015), and to study the functions of kidney pericytes in vascular stability (Schrimpf et al, 2012). This antibody has also been used in immunohistochemistry to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and to demonstrate how Bbeta(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation (Soerensen et al, 2011). In addition, in vivo applications of this agonistic anti-TIM-1 antibody (clone 3B3) has been shown, for instance, to heighten T cell activation and prevent the development of respiratory tract tolerance in a Th2-driven model of asthma (Umetsu et al, 2005), to increase the frequency of antigen-specific T cells, the production of the proinflammatory cytokines IFN-gamma and IL-17, and thus the severity of experimental autoimmune encephalomyelitis (Sheng et al, 2007), as well as to deprogram Tregs and prevent transplant tolerance in mice (Degauque et al, 2008). The agonistic effect of 3B3 is exerted by cross-linking of TIM-1 molecules, as Fab fragments of 3B3 do not show this activity (Umetsu, 2005). As this cross-linking was observed in the presence of dendritic cells, using a rat IgG2a version of theantibody, the cross-linking is likely Fc-dependet.

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

Research Use Only

Monoclonal

3B3

Havcr1

hepatitis A virus cellular receptor 1

AI503787; HAVcr-1; hepatitis A virus cellular receptor 1 homolog; kidney injury molecule 1; KIM-; KIM-1; t cell immunoglobulin and mucin domain-containing protein 1; t cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; Tim; TIM-; Tim1; TIM-1; Timd1; TIMD-1

Mouse

IgG2a

Hepatitis A virus cellular receptor 1 homolog

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203