Anti-Tim-1 [RMT1-10]

Absolute Antibody

Anti-Tim-1 [RMT1-10]

7

This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. IgV domain of Tim-1 was proposed as the epitope of RMT1-10 (Xiao et al., 2007; pmid: 17606630). RMT1-10 did not corss-react with

This anti-Tim-1 antibody inhibits the generation of antigen-specific T cells, production of IFN-gamma and IL-17, and development of autoimmunity, and it caused a strong Th2 response (Xiao et al., 2007; pmid: 17606630). RMT1-10 gives then rise to opposite effects than those brought about by another anti-Tim1 clone - 3B3 (Ab01032) which achtivates T-cells. The use of RMT1-10 demonstrated that binding Tim-1 can not only lead to activation of T-cells (as it had been known before) but that it can also cause the aforementioned inhibition (Xiao et al., 2007; pmid: 17606630). Consequently, the opposite antibodies RMT1-10 and 3B3 (Ab01032) can be utilized together to study various aspects of Tim-1 costimulation and its consequences. This antibody was used in multiple flow cytometry (FC/FACS) experimetns, such as identifying Tim-1-expressing EL-4 cells to determine the antibodys epitope (Xiao et al., 2007; pmid: 17606630). Furthermore, Tim-1 activation was suggested as an important factor in certain pathologies, and thus this inhibitory antibody can be useful for research on the potential treatment options. For instance, RMT1-10 was utilized in a research model of glomerular disease. It was demonstrated that crescentic glomerulonephritis, proliferative injury, and leukocyte accumulation were attenuated following treatment with RMT1-10, but interstitial foxp3+ cell accumulation and interleukin-10 mRNA were increased. Apoptosis and T-cell proliferation as well as Th1 and Th17 responses decreased both in the whole model organism as well as in kidney (Nozaki et al., 2012; pmid: 22205357). Another study showed that RMT1-10 attenuates atherosclerosis by expanding IgM-producing B1a cells (Hosseini et al, 2018; pmid: 29936416). Finally, another group demonstrated that this clone promotes the survival corneal allografts in mice what is associated with the increase in T regulatory cells (Tan et al., 2014; pmid: 24613782).

Flow Cytometry, ImmunoFluorescence, ELISA, Neutralisation/Blocking

Inhibiting; FC; ELISA; IF

Research Use Only

Monoclonal

RMT1-10

Havcr1

hepatitis A virus cellular receptor 1

AI503787; HAVcr-1; hepatitis A virus cellular receptor 1 homolog; kidney injury molecule 1; KIM-; KIM-1; t cell immunoglobulin and mucin domain-containing protein 1; t cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; Tim; TIM-; Tim1; TIM-1; Timd1; TIMD-1

Mouse

IgG2b

Hepatitis A virus cellular receptor 1 homolog

This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

The original rat IgG2a version of this antibody was raised by immunizing SD rats with full-length Tim-1-Ig that contained both IgV and mucin domains of Tim-1. Lymph node cells were then fused with P3U1 myeloma cells and cloned creating hybridomas.

-20°C,2°C to 8°C

12352203