Figure 1. Western blot analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tim 2/Timd2 antigen affinity purified polyclonal antibody (Catalog # A32807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tim 2/Timd2 at approximately 70 kDa. The expected band size for Tim 2/Timd2 is at 34 kDa.
Figure 1. Western blot analysis of Tim 2/Timd2 using anti-Tim 2/Timd2 antibody (A32807). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tim 2/Timd2 antigen affinity purified polyclonal antibody (Catalog # A32807) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tim 2/Timd2 at approximately 70 kDa. The expected band size for Tim 2/Timd2 is at 34 kDa.
Anti-Tim 2/Timd2 Antibody Picoband(r)
A32807-DYLIGHT488
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetTimd2
Overview
- SupplierBoster Bio
- Product NameAnti-Tim 2/Timd2 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID171284
- Target nameTimd2
- Target descriptionT cell immunoglobulin and mucin domain containing 2
- Target synonymsAA816106; AU019358; AU019662; C78111; G9D3; T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; Tim; TIM-; Tim2; TIM-2; TIMD-2
- HostRabbit
- IsotypeIgG
- Protein IDQ8R183
- Protein NameT-cell immunoglobulin and mucin domain-containing protein 2
- Scientific DescriptionBoster Bio Anti-Tim 2/Timd2 Antibody Picoband® catalog # A32807. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203