Anti-Tim-3 [2C12]

Absolute Antibody

Anti-Tim-3 [2C12]

7

This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytopl

This antibody was used in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis model to study the effects of TIM-3 triggering in dendritic cells (Anderson et al., 2007; PMID: 18006747). Furthermore, this antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al., 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al., 2010; PMID: 20819927), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al., 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al., 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al., 2010; PMID: 20819927) (Ngiow et al., 2011; PMID: 21430066).

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry, Neutralisation/Blocking

FC; IF; IHC; blocking; therapeutic

Research Use Only

Monoclonal

2C12

Havcr2

hepatitis A virus cellular receptor 2

HAVcr-2; hepatitis A virus cellular receptor 2 homolog; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; Tim; TIM-; Tim3; TIM-3; Timd; Timd3; TIMD-3

Rat

IgG1

Hepatitis A virus cellular receptor 2 homolog

Mouse

Mouse

This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund’s adjuvant.

-20°C,2°C to 8°C

12352203