Figure 1. Western blot analysis of TIM 3/HAVCR2 using anti-TIM 3/HAVCR2 antibody (A00657-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3/HAVCR2 antigen affinity purified polyclonal antibody (Catalog # A00657-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3/HAVCR2 at approximately 55 kDa. The expected band size for TIM 3/HAVCR2 is at 33 kDa.
Figure 1. Western blot analysis of TIM 3/HAVCR2 using anti-TIM 3/HAVCR2 antibody (A00657-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3/HAVCR2 antigen affinity purified polyclonal antibody (Catalog # A00657-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3/HAVCR2 at approximately 55 kDa. The expected band size for TIM 3/HAVCR2 is at 33 kDa.
Anti-TIM 3/HAVCR2 Antibody Picoband(r)
A00657-4-CY3
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHAVCR2
Overview
- SupplierBoster Bio
- Product NameAnti-TIM 3/HAVCR2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID84868
- Target nameHAVCR2
- Target descriptionhepatitis A virus cellular receptor 2
- Target synonymsCD366; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; Tim-3; TIMD3; TIMD-3
- HostRabbit
- IsotypeIgG
- Protein IDQ8TDQ0
- Protein NameHepatitis A virus cellular receptor 2
- Scientific DescriptionBoster Bio Anti-TIM 3/HAVCR2 Antibody Picoband® catalog # A00657-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203