Figure 1. Western blot analysis of TIMP3 using anti-TIMP3 antibody (PB9831). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Ovary Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (Catalog # PB9831) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 34 kDa. The expected band size for TIMP3 is at 34 kDa.
Figure 1. Western blot analysis of TIMP3 using anti-TIMP3 antibody (PB9831). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Ovary Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (Catalog # PB9831) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 34 kDa. The expected band size for TIMP3 is at 34 kDa.
Anti-TIMP3 Antibody Picoband(r)
PB9831
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTIMP3
Overview
- SupplierBoster Bio
- Product NameAnti-TIMP-3 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID7078
- Target nameTIMP3
- Target descriptionTIMP metallopeptidase inhibitor 3
- Target synonymsHSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; protein MIG-5; SFD; TIMP-3; tissue inhibitor of metalloproteinases 3
- HostRabbit
- IsotypeIgG
- Protein IDP35625
- Protein NameMetalloproteinase inhibitor 3
- Scientific DescriptionBoster Bio Anti-TIMP3 Antibody Picoband® catalog # PB9831. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Hydrogen sulfide ameliorates doxorubicin-induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway. Nie L et al., 2021 Apr, Mol Med RepRead more