Figure 1. Western blot analysis of Timp-1 using anti-Timp-1 antibody (A00561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: rat ovarian tissue lysates, Lane 4: mouse ovarian tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Timp-1 antigen affinity purified polyclonal antibody (Catalog # A00561-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Timp-1 at approximately 26KD. The expected band size for Timp-1 is at 23KD.
Figure 1. Western blot analysis of Timp-1 using anti-Timp-1 antibody (A00561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: rat ovarian tissue lysates, Lane 4: mouse ovarian tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Timp-1 antigen affinity purified polyclonal antibody (Catalog # A00561-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Timp-1 at approximately 26KD. The expected band size for Timp-1 is at 23KD.
Anti-TIMP1 Antibody Picoband(r)
A00561-1-CY3
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTIMP1
Overview
- SupplierBoster Bio
- Product NameAnti-TIMP1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID7076
- Target nameTIMP1
- Target descriptionTIMP metallopeptidase inhibitor 1
- Target synonymsCLGI; collagenase inhibitor; EPA; epididymis secretory sperm binding protein; EPO; erythroid potentiating activity; fibroblast collagenase inhibitor; HCI; metalloproteinase inhibitor 1; TIMP; TIMP-1; tissue inhibitor of metalloproteinases 1
- HostRabbit
- IsotypeIgG
- Protein IDP01033
- Protein NameMetalloproteinase inhibitor 1
- Scientific DescriptionBoster Bio Anti-TIMP1 Antibody Picoband® catalog # A00561-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203