Anti-Tissue factor [D3H44]

Absolute Antibody

Anti-Tissue factor [D3H44]

7

This antibody is the humanized and affinity matured version of murine antibody D3. This antibody binds human tissue factor with a 100-fold increased affinity (KD 0.1 nM.) as compared to the original murine and chimeric versions. In vitro, D3H44 displays potent inhibition of plasma clotting, prevents FXa activation through the TF/FVIIa pathway (IC50 = 47 pM) and prevents fibrin deposition in a human ex-vivo thrombosis model (IC50 = 37 nM.) (PMID: 11307801). In vivo studies with this antibody demonstrated efficient antithrombotic activity. Anti-TF antibodies may prove useful in cardiovascular disorders and cancer, given the role of TF in these diseases (PMID: 15379713). This antibody was used in the immunofluorescence staining of tissue factor expressed on the surface of SW480 colon cancer cells. Fibrin formation was observed in plasma after 200 secs (PMID: 30462538).

Functional Assay, ImmunoFluorescence, Western Blot, ELISA, Neutralisation/Blocking

ELISA; WB; IF; Neutralize; functional assay

Research Use Only

Tissue factor

This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203