Anti-TL1A [chTL1A-1]

Absolute Antibody

Anti-TL1A [chTL1A-1]

9

The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of

The specificity of the original antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a capture antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. The antibody identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040).

Western Blot, ELISA, ImmunoCytoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

chTL1A-1

TNFSF15

tumor necrosis factor superfamily member 15

TNF superfamily member 15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; vascular endothelial growth inhibitor

Rabbit

IgG

TL1A

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Chicken

-20°C,2°C to 8°C

12352203