Figure 1. Western blot analysis of TMEM129 using anti-TMEM129 antibody (A11755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM129 antigen affinity purified polyclonal antibody (Catalog # A11755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM129 at approximately 50 kDa. The expected band size for TMEM129 is at 40 kDa.
Figure 1. Western blot analysis of TMEM129 using anti-TMEM129 antibody (A11755). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM129 antigen affinity purified polyclonal antibody (Catalog # A11755) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM129 at approximately 50 kDa. The expected band size for TMEM129 is at 40 kDa.
Anti-TMEM129 Antibody Picoband(r)
A11755-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTMEM129
Overview
- SupplierBoster Bio
- Product NameAnti-TMEM129 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID92305
- Target nameTMEM129
- Target descriptiontransmembrane protein 129, E3 ubiquitin ligase
- Target synonymsD4S2561E; E3 ubiquitin-protein ligase TM129; RING-type E3 ubiquitin transferase TM129; transmembrane protein 129, E3 ubiquitin protein ligase
- HostRabbit
- IsotypeIgG
- Protein IDA0AVI4
- Protein NameE3 ubiquitin-protein ligase TM129
- Scientific DescriptionBoster Bio Anti-TMEM129 Antibody Picoband® catalog # A11755. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203