More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-TMPRSS3 Antibody Picoband(r)
Figure 1. Western blot analysis of TMPRSS3 using anti-TMPRSS3 antibody (A04164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS3 antigen affinity purified polyclonal antibody (Catalog # A04164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS3 at approximately 48KD. The expected band size for TMPRSS3 is at 48KD.
Figure 1. Western blot analysis of TMPRSS3 using anti-TMPRSS3 antibody (A04164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS3 antigen affinity purified polyclonal antibody (Catalog # A04164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS3 at approximately 48KD. The expected band size for TMPRSS3 is at 48KD.
Figure 1. Western blot analysis of TMPRSS3 using anti-TMPRSS3 antibody (A04164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS3 antigen affinity purified polyclonal antibody (Catalog # A04164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS3 at approximately 48KD. The expected band size for TMPRSS3 is at 48KD.

Anti-TMPRSS3 Antibody Picoband(r)

A04164-2-BIOTIN
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTMPRSS3
100 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TMPRSS3 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Biotin
  • Gene ID64699
  • Target name
    TMPRSS3
  • Target description
    transmembrane serine protease 3
  • Target synonyms
    DFNB10; DFNB8; ECHOS1; serine protease TADG-12; TADG12; transmembrane protease serine 3; transmembrane protease, serine 3; tumor-associated differentially-expressed gene 12 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP57727
  • Protein Name
    Transmembrane protease serine 3
  • Scientific Description
    Boster Bio Anti-TMPRSS3 Antibody catalog # A04164-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203