Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PB9010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC-12 Whole Cell Lysate, Lane 2: Rat Spleen Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: Rat Kidney Tissue Lysate, Lane 5: Rat Liver Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PB9010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 26KD. The expected band size for TNF alpha is at 26KD.
Figure 1. Western blot analysis of TNF alpha using anti-TNF alpha antibody (PB9010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: PC-12 Whole Cell Lysate, Lane 2: Rat Spleen Tissue Lysate, Lane 3: Rat Brain Tissue Lysate, Lane 4: Rat Kidney Tissue Lysate, Lane 5: Rat Liver Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PB9010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 26KD. The expected band size for TNF alpha is at 26KD.
Anti-TNF alpha Antibody Picoband(r)
PB9010-DYLIGHT594
ApplicationsWestern Blot
Product group Antibodies
ReactivityRat
TargetTnf
Overview
- SupplierBoster Bio
- Product NameAnti-TNF alpha Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for TNF alpha is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID24835
- Target nameTnf
- Target descriptiontumor necrosis factor
- Target synonymscachectin; RATTNF; Tnfa; TNF-alpha; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
- HostRabbit
- IsotypeIgG
- Protein IDP16599
- Protein NameTumor necrosis factor
- Scientific DescriptionBoster Bio Anti-TNF alpha Antibody Picoband® catalog # PB9010. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityRat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203