Figure 1. Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.
Figure 1. Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.
Anti-TNF Receptor II/TNFRSF1B Antibody Picoband(r)
PB9515-FITC
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetTnfrsf1b
Overview
- SupplierBoster Bio
- Product NameAnti-TNF Receptor II/TNFRSF1B Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateFITC
- Gene ID21938
- Target nameTnfrsf1b
- Target descriptiontumor necrosis factor receptor superfamily, member 1b
- Target synonymsCD120b; p7; p75; p75 TNFR; p80 TNF-alpha receptor; TN; Tnf; TNF-; TNF receptor beta chain; TNF-a; TNFal; TNF-alphaR2; TNFalpha-R2; TNFBR; TNFR; TNF-R; Tnfr-1; Tnfr2; TNF-R2; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor type II
- HostRabbit
- IsotypeIgG
- Protein IDP25119
- Protein NameTumor necrosis factor receptor superfamily member 1B
- Scientific DescriptionBoster Bio Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® catalog # PB9515. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203