Figure 1. Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 28KD. The expected band size for TNFRSF18 is at 26KD.
Figure 1. Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 28KD. The expected band size for TNFRSF18 is at 26KD.
Anti-TNFRSF18 Antibody Picoband(r)
A03125-1-DYLIGHT488
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNFRSF18
Overview
- SupplierBoster Bio
- Product NameAnti-TNFRSF18 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 488
- Gene ID8784
- Target nameTNFRSF18
- Target descriptionTNF receptor superfamily member 18
- Target synonymsactivation-inducible TNFR family receptor; AITR; CD357; ENERGEN; GITR; GITR-D; glucocorticoid-induced TNFR-related protein; TNF receptor superfamily activation-inducible protein; tumor necrosis factor receptor superfamily member 18
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y5U5
- Protein NameTumor necrosis factor receptor superfamily member 18
- Scientific DescriptionBoster Bio Anti-TNFRSF18 Antibody Picoband® catalog # A03125-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203