Figure 1. Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa.
Figure 1. Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa.
Anti-TOMM70 Antibody Picoband(r)
A09172-2-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTOMM70
Overview
- SupplierBoster Bio
- Product NameAnti-TOMM70 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID9868
- Target nameTOMM70
- Target descriptiontranslocase of outer mitochondrial membrane 70
- Target synonymsmitochondrial import receptor subunit TOM70; mitochondrial precursor proteins import receptor; Tom70; TOMM70A; translocase of outer membrane 70 kDa subunit; translocase of outer mitochondrial membrane 70 homolog A; translocase of outer mitochondrial membrane protein 70
- HostRabbit
- IsotypeIgG
- Protein IDO94826
- Protein NameMitochondrial import receptor subunit TOM70
- Scientific DescriptionBoster Bio Anti-TOMM70 Antibody Picoband® catalog # A09172-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203